TBXA2R Polyklonaler Antikörper

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• KD/KO Validated

TBXA2R Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 27159-1-AP

Synonyme

Prostanoid TP receptor, TBXA2R, thromboxane A2 receptor, TP, TXA2 R

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Galerie der Validierungsdaten

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  WB analysis using 27159-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 27159-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 27159-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 27159-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 27159-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, DU 145-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

27159-1-AP bindet in WB, IHC, ELISA TBXA2R und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBXA2R fusion protein Ag25670
• Vollständiger Name: thromboxane A2 receptor
• Berechnetes Molekulargewicht: 343aa,37 kDa; 1043aa,113 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC074750
• Gene symbol: TBXA2R
• Gene ID (NCBI): 6915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBXA2R, also named as thromboxane A2 receptor, TXA2-R and Prostanoid TP receptor, belongs to G-protein coupled receptor 1 family. Thromboxane A2 receptor is a key molecule in hemostasis as its abnormality leads to bleeding disorders. The TXA2-R receptor is linked via a guanine nucleotide-binding protein (G protein) to phospholipase C (PLC), which hydrolyses phosphoinositides to the two potent stimulatory second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol. IP3 causes increases in cytoplasmic free calcium and diacylglycerol causes activation of protein kinase C. In the kidney, the binding of TXA2-R to glomerular TP receptors causes intense vasoconstriction. The two isoforms expressed in cultured cells show similar ligand binding characteristics and phospholipase C (PLC) activation but oppositely regulate adenylyl cyclase activity (PMID: 8613548).

Publikationen

Species	Application	Title
mouse	WB	Am J Respir Crit Care Med; Thromboxane-Prostanoid Receptor Signaling Drives Persistent Fibroblast Activation in Pulmonary Fibrosis; Authors - Toshio Suzuki ; KO Validated
rat	WB, IHC	Front Endocrinol (Lausanne); The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats.; Authors - Meihe Li