TBX3 Polyklonaler Antikörper

TBX3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 16741-1-AP

Synonyme

T box 3, T box protein 3, TBX3, TBX3 ISO, UMS, XHL

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 16741-1-AP

  IP Result of anti-TBX3 (IP:16741-1-AP, 4ug; Detection:16741-1-AP 1:500) with human placenta tissue lysate 1520ug.

• 

  IHC staining of human thyroid cancer using 16741-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16741-1-AP (TBX3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 16741-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16741-1-AP (TBX3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 4 publications below

Produktinformation

16741-1-AP bindet in WB, IP, IHC, ELISA TBX3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBX3 fusion protein Ag9955
• Vollständiger Name: T-box 3
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC025258
• Gene symbol: TBX3
• Gene ID (NCBI): 6926
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBX3, also named as T-box protein 3, is a 743 amino acid protein, which contains 1 T-box DNA-binding domain. TBX3 may localize in the nucleus and is widely expressed in various tissues. TBX3 as a transcriptional repressor is involved in developmental processes and probably plays a role in limb pattern formation. TBX3 plays an important role on osteogenic differentiation and proliferation of human mesenchymal stem cell derived from adipose tissue.

Publikationen

Species	Application	Title
	IHC	Nat Commun; Targeting myeloid derived suppressor cells reverts immune suppression and sensitizes BRAF-mutant papillary thyroid cancer to MAPK inhibitors.; Authors - Peitao Zhang
marmoset	WB	Int J Mol Med; Leukemia inhibitory factor regulates marmoset induced pluripotent stem cell proliferation via a PI3K/‌Akt‑dependent Tbx‑3 activation pathway.; Authors - Minxia Ke
human	WB,IHC	Cell Signal; A new PKCα/β/TBX3/E-cadherin pathway is involved in PLCε-regulated invasion and migration in human bladder cancer cells.; Authors - Du Hong Fei HF
human	IHC	Hum Genomics; Identification of TBX2 and TBX3 variants in patients with conotruncal heart defects by target sequencing.; Authors - Huilin Xie
human	WB,IHC	Exp Cell Res; HOTAIR maintains the stemness of ovarian cancer stem cells via the miR-206/TBX3 axis.; Authors - Yifan Zhang
	WB	Genesis; Homologous recombination efficiency enhanced by inhibition of MEK and GSK3β; Authors - Zhaoyu Lin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Li (Verified Customer) (12-03-2018)

• Applications: Western Blot, Immunohistochemistry,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human cell lines and mouse liver tissue

TBX3 Antibody Western Blot,Immunohistochemistry, validation (1:1000 dilution) in human cell lines and mouse liver tissue (Cat no:16741-1-AP)