TBC1D5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TBC1D5 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (3)

Kat-Nr. : 17078-1-AP

Synonyme

KIAA0210, TBC1 domain family member 5, TBC1 domain family, member 5, TBC1D5

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Galerie der Validierungsdaten

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  WB analysis using 17078-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17078-1-AP (TBC1D5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17078-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17078-1-AP (TBC1D5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17078-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17078-1-AP (TBC1D5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 17078-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 17078-1-AP (TBC1D5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 17078-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 17078-1-AP (TBC1D5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 17078-1-AP

  IP Result of anti-TBC1D5 (IP:17078-1-AP, 4ug; Detection:17078-1-AP 1:300) with A431 cells lysate 2400ug.

• 

  IHC staining of mouse testis using 17078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17078-1-AP (TBC1D5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17078-1-AP (TBC1D5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, A431-Zellen, Jurkat-Zellen, PC-3-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IF: See 3 publications below

Produktinformation

17078-1-AP bindet in WB, IP, IHC, IF, ELISA TBC1D5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBC1D5 fusion protein Ag10991
• Vollständiger Name: TBC1 domain family, member 5
• Berechnetes Molekulargewicht: 795 aa, 89 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: BC013145
• Gene symbol: TBC1D5
• Gene ID (NCBI): 9779
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBC1D5, a member of TBC (Tre2/Bub2/Cdc16)1 domain family, is a novel retromer-interacting protein. TBC1D5 is a Rab GTPase-activating proteins (GAPs) proteins that negatively regulates VPS35/29/26 recruitment and causes Rab7 to dissociate from the membrane. TBC1D5 could bridge the endosome and autophagosome via its C-terminal LIR motif, and Rab GAPs are implicated in the reprogramming of the endocytic trafficking events under starvation-induced autophagy. The TBC1D5 protein exists 89 kDa and 91 kDa isoforms.

Publikationen

Species	Application	Title
human	WB,IF	Sci Adv; De novo macrocyclic peptides for inhibiting, stabilizing, and probing the function of the retromer endosomal trafficking complex.; Authors - Kai-En Chen
human	WB	EMBO J; Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy.; Authors - Ana Jimenez-Orgaz ; KO Validated
human	WB	J Cell Biol; Retromer and TBC1D5 maintain late endosomal RAB7 domains to enable amino acid-induced mTORC1 signaling.; Authors - Arunas Kvainickas ; KO Validated
	WB	EMBO Rep; BioID reveals an ATG9A interaction with ATG13-ATG101 in the degradation of p62/SQSTM1-ubiquitin clusters.; Authors - Ashari R Kannangara
human	WB	Int J Biol Sci; TBK1 Facilitates GLUT1-Dependent Glucose Consumption by suppressing mTORC1 Signaling in Colorectal Cancer Progression.; Authors - Diyuan Zhou ; KD Validated
mouse	IF	Mol Biol Cell; Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.; Authors - Gomez Timothy S TS

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

q (Verified Customer) (06-01-2021)

It is OK to detect TBC1D5 (100 kD) in both mouse and postmortem brain lysates, although there are some non specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain and postmortembrain lysates

TBC1D5 Antibody Western Blot validation (1:3000 dilution) in mouse brain and postmortembrain lysates (Cat no:17078-1-AP)

FH

Florian (Verified Customer) (04-09-2019)

This is a very strong antibody that is reasonably specific in western blot applications. We have validated it through Crispr/Cas9 mediated knockout of TBC1D5 in HeLa cells (Jimenez-Orgaz et al, EMBOJ, 2018). While the strongest band is indeed TBC1D5, as our knockout confirms, it also produces several weaker, unspecific bands on membranes blocked with 5% milk. Because of the unspecific bands, I rate it with four stars instead of five. We have not tested it for immunofluorescence. Overall, it is a very good antibody that will probably also work at much lower dilutions than 1:1000. Diluting it further may also reduce the unspecific signal.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela cells

FH

David (Verified Customer) (02-28-2019)

Hela cells stained with anti-TBC1D5 (green) and Hoechst (blue)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Hela cells

TBC1D5 Antibody Western Blot,Immunofluorescence, validation (1:200 dilution) in Hela cells (Cat no:17078-1-AP)