TBC1D23 Polyklonaler Antikörper

TBC1D23 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (2)

Kat-Nr. : 17002-1-AP

Synonyme

NS4ATP1, TBC1 domain family member 23, TBC1 domain family, member 23, TBC1D23

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 17002-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17002-1-AP (TBC1D23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17002-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17002-1-AP (TBC1D23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17002-1-AP

  IP Result of anti-TBC1D23 (IP:17002-1-AP, 4ug; Detection:17002-1-AP 1:500) with HepG2 cells lysate 2400ug.

• 

  IHC staining of human liver using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17002-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17002-1-AP (TBC1D23 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 17002-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 17002-1-AP (TBC1D23 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Eierstockgewebe, humanes Hodengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

17002-1-AP bindet in WB, IP, IHC, IF, ELISA TBC1D23 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBC1D23 fusion protein Ag10697
• Vollständiger Name: TBC1 domain family, member 23
• Berechnetes Molekulargewicht: 699 aa, 78 kDa
• Beobachtetes Molekulargewicht: 70-78 kDa
• GenBank-Zugangsnummer: BC010221
• Gene symbol: TBC1D23
• Gene ID (NCBI): 55773
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBC1D23, also named as NS4ATP1, is a gene down-regulated by Pneumocystis infection. Pneumocystis pneumonia is a common opportunistic disease in AIDS patients. (PMID: 20377877) Recently it has been found that TBC1D23 gene is the most frequently mutated in MSI-H cell lines and protein expressions of TBC1D23 in tumors with mutation were down regulated.(PMID: 20824714) This antibody is specific to TBC1D23.

Publikationen

Species	Application	Title
human	WB,IF	Am J Hum Genet; Homozygous Mutations in TBC1D23 Lead to a Non-degenerative Form of Pontocerebellar Hypoplasia.; Authors - Isaac Marin-Valencia
human	IF	Am J Hum Genet; Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development.; Authors - Ekaterina L Ivanova
human	WB,IHC	Int J Cancer; Identification of frequently mutated genes with relevance to nonsense mediated mRNA decay in the high microsatellite instability cancers.; Authors - Shin Nara N
human	IHC,WB,IF	J Cell Mol Med; TBC1 domain family member 23 interacts with Ras-related protein Rab-11A to promote poor prognosis of non-small-cell lung cancer via β1-integrin.; Authors - Yao Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christine (Verified Customer) (01-27-2023)

Main band detected below the 85 kDa marker (expected size of TBC1D23 is 78, observed is 70-78).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293T

FH

Jerome (Verified Customer) (05-22-2019)

Most cells show the expected Golgi localisation; but not all. High background

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa cells

TBC1D23 Antibody Immunofluorescence, validation (1:100 dilution) in HeLa cells (Cat no:17002-1-AP)