TBC1D15 Polyklonaler Antikörper

TBC1D15 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17252-1-AP

Synonyme

TBC1 domain family member 15, Rab7-GAP, Rab7 GAP, GTPase-activating protein RAB7, GTPase activating protein RAB7

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  WB analysis using 17252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17252-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TBC1D15 antibody (17252-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HEK-293T-Zellen, HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

17252-1-AP bindet in WB, IF/ICC, ELISA TBC1D15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBC1D15 fusion protein Ag10625
• Vollständiger Name: TBC1 domain family, member 15
• Berechnetes Molekulargewicht: 691 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC028352
• Gene symbol: TBC1D15
• Gene ID (NCBI): 64786
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBC1D15 is a highly conserved protein belonging to the TBC domain family and has similar functions to Rab-GAP. TBC1D15 is ubiquitously expressed and localized predominantly to the cytosol. TBC1D15 is a protein that contains a conserved Tre2/Bub2/Cdc16 (TBC) domain. TBC1D15 can serve as a mitochondrial Rab GTPase-activating protein (Rab-GAP) and influence autophagosome biogenesis and morphology downstream of Parkin activation. TBC1D15 can also control stem cell self-renewal when coupled with the Numb-p53 complex.

Publikationen

Species	Application	Title
human	IF	Cell Death Dis; SENP3-FIS1 axis promotes mitophagy and cell survival under hypoxia; Authors - Alice Zhao
human	WB	J Hazard Mater; PFOS causes lysosomes-regulated mitochondrial fission through TRPML1-VDAC1 and oligomerization of MCU/ATP5J2; Authors - Wei Yang
		Aging Dis; TBC1D15 Inhibits Autophagy of Microglia through Maintaining the Damaged Swelling Lysosome in Alzheimer's Disease; Authors - You Wu