WWTR1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

WWTR1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

CloneNo.

2A12A10

Kat-Nr. : 66500-1-Ig

Synonyme

TAZ, WWTR1

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Galerie der Validierungsdaten

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  WB analysis using 66500-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66500-1-Ig

  WB result of TAZ antibody (66500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAZ transfected HepG2 cells.

• 

  WB analysis using 66500-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66500-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66500-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66500-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 66500-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TAZ antibody (66500-1-Ig, Clone: 2A12A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 4T1-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, MDA-MB-453s-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

66500-1-Ig bindet in WB, IHC, IF, ELISA WWTR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: WWTR1 fusion protein Ag13330
• Vollständiger Name: WW domain containing transcription regulator 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC014052
• Gene symbol: WWTR1
• Gene ID (NCBI): 25937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma; Authors - Yu-Fei Cao
human	WB	Nat Commun; LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.; Authors - Huai-Bin Hu ; KD Validated
mouse	WB,IHC,IF	Front Pharmacol; TAZ Induces Migration of Microglia and Promotes Neurological Recovery After Spinal Cord Injury.; Authors - Xuyang Hu
human	WB	Am J Transl Res; VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro.; Authors - Adheesh Bhandari
rat	WB,IF	Toxicol Appl Pharmacol; Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif; Authors - Xinghe Chen
human	WB,IHC	Stem Cells Dev; Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.; Authors - Shaojun Nong