TDP-43 Polyklonaler Antikörper

TDP-43 Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 18280-1-AP

Synonyme

ALS10, TAR DNA binding protein, TAR DNA binding protein 43

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 18280-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18280-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 18280-1-AP

  IP Result of anti-TARDBP (IP:18280-1-AP, 3ug; Detection:18280-1-AP 1:1500) with K-562 cells lysate 6000ug.

• 

  IHC staining of human gliomas using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18280-1-AP (TDP-43 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18280-1-AP (TDP-43 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18280-1-AP (TDP-43 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat brain using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 18280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 18280-1-AP (TDP-43 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 18280-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug TDP-43 (full length) antibody (18280-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

18280-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TDP-43 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: TAR DNA binding protein
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC001487
• Gene symbol: TARDBP
• Gene ID (NCBI): 23435
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues.

Publikationen

Species	Application	Title
		Nat Neurosci; TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.; Authors - Florent Laferrière
human	IHC	Acta Neuropathol Commun; Anatomic survey of seeding in Alzheimer's disease brains reveals unexpected patterns.; Authors - Barbara E Stopschinski
human	WB	Neurobiol Dis; Tau-tubulin kinase 1 phosphorylates TDP-43 at disease-relevant sites and exacerbates TDP-43 pathology; Authors - Yuan Tian
mouse	IF	Dis Model Mech; Viral delivery of C9ORF72 hexanucleotide repeat expansions in mice lead to repeat length dependent neuropathology and behavioral deficits.; Authors - Saul Herranz-Martin
human	IHC	J Neurotrauma; Multimodal Biomarkers of Repetitive Head Impacts and Traumatic Encephalopathy Syndrome: A Clinico-Pathological Case Series.; Authors - Breton Asken
human	IHC	J Alzheimers Dis; Pathological Correlates of Cognitive Impairment in The University of Manchester Longitudinal Study of Cognition in Normal Healthy Old Age.; Authors - Andrew C Robinson