TAOK1 Polyklonaler Antikörper

TAOK1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 26250-1-AP

Synonyme

hKFC B, KIAA1361, Kinase from chicken homolog B, MAP3K16, MARKK, PSK2, STE20 like kinase PSK2, TAO kinase 1, TAO1, TAOK1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 26250-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26250-1-AP (TAOK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26250-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26250-1-AP (TAOK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26250-1-AP (TAOK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26250-1-AP (TAOK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 26250-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26250-1-AP (TAOK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 5 publications below

Produktinformation

26250-1-AP bindet in WB, IHC, IF, ELISA TAOK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAOK1 fusion protein Ag22461
• Vollständiger Name: TAO kinase 1
• Berechnetes Molekulargewicht: 1001 aa, 116 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC133039
• Gene symbol: TAOK1
• Gene ID (NCBI): 57551
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thousand and one kinase 1 (TAOK1), also known as prostate-derived sterile 20 (Ste20)-like kinase 2, TAO1 (thousand and one amino-acid protein 1) or MARKK, is a member of the MAP3K protein kinase and belongs to the germinal-center kinase-like class of sterile 20 (Ste20)-like kinases (PMID: 29400705). TAOK1 is involved in mammalian brain development and functions as a negative regulator in IL-17-mediated signaling and inflammation (PMID: 33565190). TAOK1 has a calculated molecular mass of 116 kDa and encodes a serine/threonine protein kinase at its N terminus.

Publikationen

Species	Application	Title
human	WB,IF	Am J Hum Genet; Variants in EXOSC9 Disrupt the RNA Exosome and Result in Cerebellar Atrophy with Spinal Motor Neuronopathy.; Authors - David T Burns
human	WB,IF	Acta Neuropathol Commun; A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies.; Authors - Caterina Giacomini
mouse	IF	Sci Signal; Neurodevelopmental disorder-associated mutations in TAOK1 reveal its function as a plasma membrane remodeling kinase; Authors - Neal Beeman
human	WB,IF	Mol Cancer Ther; Targeting TAO kinases using a new inhibitor compound delays mitosis and induces mitotic cell death in centrosome amplified breast cancer cells.; Authors - Chuay-Yeng Koo
monkey	WB	J Cell Sci; Rnd3 interacts with TAO kinases and contributes to mitotic cell rounding and spindle positioning.; Authors - Ritu Garg
human	WB,IF	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj