Neurokinin-1 receptor Polyklonaler Antikörper

Neurokinin-1 receptor Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 17942-1-AP

Synonyme

Neurokinin-1 receptor, NK 1 receptor, NK 1R, NK1R, NK-1R, NKIR, SPR, Substance P receptor, TAC1R, tachykinin receptor 1, TACR1

---

Galerie der Validierungsdaten

• 

  WB analysis using 17942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17942-1-AP (Neurokinin-1 receptor antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17942-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17942-1-AP (Neurokinin-1 receptor antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 17942-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 17942-1-AP (Neurokinin-1 receptor antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 17942-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17942-1-AP (Neurokinin-1 receptor antibody at dilution of 1:300 incubated at 4 degree celsius over night.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Caco-2-Zellen, HeLa-Zellen, Maus-Pankreasgewebe, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

17942-1-AP bindet in WB, ELISA Neurokinin-1 receptor und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Neurokinin-1 receptor fusion protein Ag12368
• Vollständiger Name: tachykinin receptor 1
• Berechnetes Molekulargewicht: 407 aa, 46 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC074911
• Gene symbol: TACR1
• Gene ID (NCBI): 6869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

neurokinin 1 receptor (NK1R, also known as the tachykinin 1 receptor, TACR1), a mediator of behavioral stress responses, in alcohol dependence and treatment. One such neurotransmitter is substance P (SP), which together with its preferred TACR1 is highly expressed in brain areas involved in stress responses and drug reward, including the hypothalamus, amygdala, and nucleus accumbens (PMID: 18276852).

Publikationen

Species	Application	Title
rat	WB	Phytomedicine; Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.; Authors - Ping Ma
mouse	WB	PLoS One; Hemokinin-1(4-11)-Induced Analgesia Selectively Up-Regulates δ-Opioid Receptor Expression in Mice.; Authors - Cai-Yun Fu
mouse	WB	J Neuroimmunol; Tropisetron attenuates lipopolysaccharide induced neuroinflammation by inhibiting NF-κB and SP/NK1R signaling pathway.; Authors - Yan Yu
mouse	WB	Peptides; Study on the distribution sites and the molecular mechanism of analgesia after intracerebroventricular injection of rat/mouse hemokinin-1 in mice.; Authors - Xia Rui-Long RL
human	WB	Neoplasma; miR-877-5p antagonizes the promoting effect of SP on the gastric cancer progression.; Authors - Xiao-Dan Guo
human	WB	Pharm Biol; Stigmasterol alleviates allergic airway inflammation and airway hyperresponsiveness in asthma mice through inhibiting substance-P receptor; Authors - Jimei Zhang