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Synaptotagmin-1 Monoklonaler Antikörper

Synaptotagmin-1 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H1H2

Kat-Nr. : CL488-68043

Synonyme

DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, SYT, SYT1, SytI

Galerie der Validierungsdaten

Filter:
  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 488-conjugated Synaptotagmin-1 Mono (CL488-68043)

    FC experiment of SH-SY5Y using CL488-68043

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Synaptotagmin-1 (CL488-68043, Clone:1H1H2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCSH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68043 bindet in FC (Intra) Synaptotagmin-1 und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

Getestete Reaktivität Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Synaptotagmin-1 fusion protein Ag6071
Vollständiger Name synaptotagmin I
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht60-65 kDa
GenBank-ZugangsnummerBC058917
Gene symbol SYT1
Gene ID (NCBI) 6857
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 488 Synaptotagmin-1 antibody CL488-68043Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen