Sortilin Monoklonaler Antikörper

Sortilin Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B11B9

Kat-Nr. : 68007-1-Ig

Synonyme

100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3, SORT1, Sortilin, sortilin 1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 68007-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68007-1-Ig (Sortilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 68007-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 68007-1-Ig (SORT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 68007-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68007-1-Ig (SORT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 68007-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68007-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68007-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68007-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 68007-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 68007-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of SH-SY5Y using 68007-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Hausschwein-Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68007-1-Ig bindet in WB, IHC, IF, ELISA Sortilin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Sortilin fusion protein Ag28043
• Vollständiger Name: sortilin 1
• Berechnetes Molekulargewicht: 831 aa, 92 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC023542
• Gene symbol: SORT1
• Gene ID (NCBI): 6272
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.