Sodium iodide symporter Polyklonaler Antikörper

Sodium iodide symporter Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-24324

Synonyme

Na(+)/I( ) cotransporter, Na(+)/I( ) symporter, NIS, SLC5A5, Sodium iodide symporter, Sodium/iodide cotransporter

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Galerie der Validierungsdaten

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  FC experiment of MCF-7 using CL488-24324

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Sodium iodide symporter (CL488-24324) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-24324 bindet in FC (Intra) Sodium iodide symporter und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Sodium iodide symporter fusion protein Ag19504
• Vollständiger Name: solute carrier family 5 (sodium iodide symporter), member 5
• Berechnetes Molekulargewicht: 643 aa, 69 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa, 75-100 kDa
• GenBank-Zugangsnummer: BC105047
• Gene symbol: SLC5A5
• Gene ID (NCBI): 6528
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The sodium iodide symporter (Na+/I - symporter, NIS), encoded by SLC5A5, is an integral plasma membrane glycoprotein that plays an important role in iodide uptake by thyroid cells. Expression of sodium iodide symporter has also been found in extra-thyroidal tissues, including gastric mucosa, lactating mammary gland and salivary glands. Increased expression of sodium iodide symporter has been found in thyroid tissue from patients with Graves' disease as well as papillary thyroid carcinomas. In addition, sodium iodide symporter was found to express in majority of breast cancer tissue but not in normal tissue. Sodium iodide symporter can be a promising diagnostic and therapeutic tool for thyroid cancer and breast cancer. This antibody recognizes the mature approximately 75-100 kDa protein and a partially glycosylated 50-55 kDa protein. (PMID: 12588808, 9525971)

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C