RISC Monoklonaler Antikörper

RISC Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1C6A7

Kat-Nr. : 60086-1-Ig

Synonyme

2410018F01Rik, 4833411K15Rik, AI957256, Risc, Scpep1, serine carboxypeptidase 1

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Galerie der Validierungsdaten

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  WB analysis of human testis using 60086-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 60086-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 60086-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 60086-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 60086-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60086-1-Ig (RISC antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60086-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60086-1-Ig (RISC antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C6 using 60086-1-Ig

  Immunofluorescent analysis of C6 cells using 60086-1-Ig (RISC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, humanes Herzgewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

60086-1-Ig bindet in WB, IHC, IF RISC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RISC fusion protein Ag0614
• Vollständiger Name: serine carboxypeptidase 1
• Berechnetes Molekulargewicht: 452 aa, 51 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa/50-60 kDa
• GenBank-Zugangsnummer: AF330052
• Gene symbol: Scpep1
• Gene ID (NCBI): 74617
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Scpep1(Retinoid-inducible serine carboxypeptidase) is a 51 kDa protein which is also named as RISC and belongs to the peptidase S10 family. This protein may be involved in vascular wall and kidney homeostasis.

Publikationen

Species	Application	Title
human	WB	Autophagy; Donor MSCs release apoptotic bodies to improve myocardial infarction via autophagy regulation in recipient cells.; Authors - Huan Liu