SYNJ1 Polyklonaler Antikörper

SYNJ1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 24677-1-AP

Synonyme

INPP5G, KIAA0910, synaptojanin 1, SYNJ1

---

Galerie der Validierungsdaten

• 

  WB analysis using 24677-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24677-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 24677-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat skeletal muscle using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 24677-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 24677-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 24677-1-AP (SYNJ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Skelettmuskelgewebe, Rattenhirngewebe, Ratten-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

24677-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA SYNJ1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SYNJ1 fusion protein Ag20364
• Vollständiger Name: synaptojanin 1
• Berechnetes Molekulargewicht: 1612 aa, 178 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC136603
• Gene symbol: SYNJ1
• Gene ID (NCBI): 8867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Synaptojanin 1, a polyphosphoinositide phosphatase, is expressed as two major alternatively spliced isoforms of 145 kDa (SJ145) and 170 kDa (SJ170), which are thought to have pleiotropic roles in endocytosis, signaling and actin function. SJ145 is highly enriched in nerve terminals where it participates in clathrin-dependent synaptic vesicle recycling. SJ170, which differs from SJ145 by the presence of a carboxy-terminal extension, is the predominant isoform in developing neurons and is expressed in a variety of tissues.(PMID:10801423).

Publikationen

Species	Application	Title
human	WB	Cell Chem Biol; Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome.; Authors - David Furkert
mouse	WB,CoIP	Behav Brain Res; SYNJ1 rescues motor functions in hereditary and sporadic Parkinson's disease mice by upregulating TSP-1 expression; Authors - Yueqin Tian
mouse	IHC	Ren Fail; Network pharmacology and molecular docking of endogenous active metabolites in diabetic kidney disease; Authors - Xinmiao Xie
mouse	WB,IP	Cell Signal; Identification of novel PCTAIRE-1/CDK16 substrates using a chemical genetic screen.; Authors - Saifeldin N Shehata