SYNGAP1 Polyklonaler Antikörper

SYNGAP1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 19739-1-AP

Synonyme

KIAA1938, MRD5, Neuronal RasGAP, RASA1, RASA5, Synaptic Ras GAP 1, SYNGAP, SYNGAP1

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Galerie der Validierungsdaten

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  WB analysis using 19739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19739-1-AP (SYNGAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19739-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19739-1-AP

  IP Result of anti-SYNGAP1 (IP:19739-1-AP, 4ug; Detection:19739-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 19739-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19739-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19739-1-AP (SYNGAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 19739-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 19739-1-AP (SYNGAP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, rat brain tissues, pig brain tissues
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

19739-1-AP bindet in WB, IP, IHC, IF, ELISA SYNGAP1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: synaptic Ras GTPase activating protein 1 homolog (rat)
• Berechnetes Molekulargewicht: 1343 aa, 148 kDa
• Beobachtetes Molekulargewicht: 148 kDa
• GenBank-Zugangsnummer: NM_006772
• Gene symbol: SYNGAP1
• Gene ID (NCBI): 8831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYNGAP1, also named as KIAA1938, is the major constituent of the PSD essential for postsynaptic signaling. It's an inhibitory regulator of the Ras-cAMP pathway. SYNGAP1 is a member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. SYNGAP1 regulates AMPAR-mediated miniature excitatory postsynaptic currents. SYNGAP1 may be involved in certain forms of brain injury, leading to long-term learning and memory deficits Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5).

Publikationen

Species	Application	Title
mouse	WB	Front Aging Neurosci; Impaired Learning and Memory Ability Induced by a Bilaterally Hippocampal Injection of Streptozotocin in Mice: Involved With the Adaptive Changes of Synaptic Plasticity.; Authors - Cong-Cong Qi
mouse	WB	Neurochem Int; Resveratrol ameliorates learning and memory impairments induced by bilateral hippocampal injection of streptozotocin in mice.; Authors - Ya-Dong Wei
mouse	WB,IHC	Eur J Neurosci; SynGAP is expressed in the murine suprachiasmatic nucleus and regulates circadian-gated locomotor activity and light-entrainment capacity.; Authors - Sydney Aten
mouse	WB	Neuroscience; Further Studies on the Role of BTBD9 in the Cerebellum, Sleep-like Behaviors and the Restless Legs Syndrome; Authors - Shangru Lyu
mouse	WB	Mol Neurobiol; SNX17 Mediates Dendritic Spine Maturation via p140Cap; Authors - Qiuyan Cui
mouse	WB	J Nutr Biochem; Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline; Authors - Ping Lu