SCP3 Polyklonaler Antikörper

SCP3 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (2)

Kat-Nr. : 23024-1-AP

Synonyme

COR1, SCP 3, SCP3, SYCP3, synaptonemal complex protein 3

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Galerie der Validierungsdaten

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  WB analysis using 23024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23024-1-AP (SCP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23024-1-AP

  Rat testis tissue were subjected to SDS PAGE followed by western blot with 23024-1-AP (SCP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23024-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23024-1-AP (SCP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 23024-1-AP

  IP Result of anti-SCP3 (IP:23024-1-AP, 3ug; Detection:23024-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IF Staining of HepG2 using 23024-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23024-1-AP (SCP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HepG2-Zellen, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 14 publications below

Produktinformation

23024-1-AP bindet in WB, IP, IHC, IF, ELISA SCP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCP3 fusion protein Ag19330
• Vollständiger Name: synaptonemal complex protein 3
• Berechnetes Molekulargewicht: 236 aa, 28 kDa
• Beobachtetes Molekulargewicht: 30 kDa, 33 kDa
• GenBank-Zugangsnummer: BC062662
• Gene symbol: SYCP3
• Gene ID (NCBI): 50511
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SYCP3 is an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. SYCP3 is required for centromere pairing during meiosis in male germ cells. SYCP3 is also required for normal meiosis during spermatogenesis and male fertility. Mutations in SYCP3 are associated with azoospermia in males and susceptibility to pregnancy loss in females.

Publikationen

Species	Application	Title
mouse	WB,IF	Nucleic Acids Res; SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.; Authors - Yongjuan Guan
mouse	IHC	Genes Dev; Control of homologous recombination by the HROB-MCM8-MCM9 pathway.; Authors - Nicole Hustedt
mouse	IF	Cell Death Differ; NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.; Authors - Bin Zhang
mouse	WB,IF	Int J Nanomedicine; Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway.; Authors - Yang Fu
mouse,rat	WB	Biomed Pharmacother; Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway.; Authors - Yang Fu
mouse	IF	Andrology; Exposure to elevated temperature affects the expression of piwi-interacting RNAs and associated transcripts in mouse testes; Authors - Wenbin Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Feng-Qian (Verified Customer) (04-03-2020)

It nicely detects meiotic chromosomes on methanol-fixed fresh frozen mouse testis sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse testis

FH

Huizhong (Verified Customer) (08-31-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: mouse testis cells

SCP3 Antibody Immunofluorescence, validation (1:50 dilution) in mouse testis cells (Cat no:23024-1-AP)