SURVIVIN Monoklonaler Antikörper

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• KD/KO Validated

SURVIVIN Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

3B9H7

Kat-Nr. : 66495-1-Ig

Synonyme

API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, BIRC5, EPR 1, IAP4, SURVIVIN

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Galerie der Validierungsdaten

• 

  WB analysis using 66495-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66495-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 66495-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Jurkat using 66495-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human SURVIVIN (66495-1-Ig, Clone:3B9H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen, U-937-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Produktinformation

66495-1-Ig bindet in WB, IF, FC, ELISA SURVIVIN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SURVIVIN fusion protein Ag20958
• Vollständiger Name: baculoviral IAP repeat-containing 5
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC008718
• Gene symbol: BIRC5
• Gene ID (NCBI): 332
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

Publikationen

Species	Application	Title
human	WB	Bioeng Transl Med; Multistage targeting and dual inhibiting strategies based on bioengineered tumor matrix microenvironment-mediated protein nanocages for enhancing cancer biotherapy.; Authors - Fabiao Hu
human	WB	Exp Mol Med; m 6 A-mediated upregulation of AC008 promotes osteoarthritis progression through the miR-328-3p‒AQP1/ANKH axis; Authors - Jiashu Yang
human	WB,IF	Acta Biochim Biophys Sin (Shanghai); Proliferation inhibition and apoptosis promotion by dual silencing of VEGF and Survivin in human osteosarcoma.; Authors - Junquan Gu ; KD Validated
mouse	WB	Front Oncol; Synergistic effects of nab-PTX and anti-PD-1 antibody combination against lung cancer by regulating the Pi3K/AKT pathway through the Serpinc1 gene; Authors - Jun Zhang
human	WB	Front Pharmacol; Analysis of regulating activities of 5'-epiequisetin on proliferation, apoptosis, and migration of prostate cancer cells in vitro and in vivo; Authors - Xueni Wang
human	WB	BMC Cancer; The survivin-ran inhibitor LLP-3 decreases oxidative phosphorylation, glycolysis and growth of neuroblastoma cells; Authors - Celimene Galiger