SUPT7L Polyklonaler Antikörper

SUPT7L Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25606-1-AP

Synonyme

Adenocarcinoma antigen ART1, ART1, KIAA0764, SPT7L, STAF65, STAF65(gamma), STAF65gamma, STAGA complex 65 subunit gamma, Suppressor of Ty 7 like, SUPT7L

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 25606-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25606-1-AP (SUPT7L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25606-1-AP (SUPT7L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 25606-1-AP

  Immunofluorescent analysis of HeLa cells using 25606-1-AP (SUPT7L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Gliomgewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25606-1-AP bindet in WB, IHC, IF,ELISA SUPT7L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SUPT7L fusion protein Ag22264
• Vollständiger Name: suppressor of Ty 7 (S. cerevisiae)-like
• Berechnetes Molekulargewicht: 414 aa, 46 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC074000
• Gene symbol: SUPT7L
• Gene ID (NCBI): 9913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SUPT7L, also named as KIAA0764, STAF65gamma, is a component of the STAGA transcription coactivator-HAT complex. The MW of SUPT7L is 46-65 kDa.