SULT1A3 Polyklonaler Antikörper

SULT1A3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 19499-1-AP

Synonyme

Aryl sulfotransferase 1A3/1A4, HAST, HAST3, M PST, ST1A3/ST1A4, ST1A5, STM, Sulfotransferase 1A3/1A4, SULT1A3, SULT1A4, TL PST

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Galerie der Validierungsdaten

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  WB analysis using 19499-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19499-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 19499-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19499-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19499-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19499-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 19499-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 19499-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 19499-1-AP

  IP Result of anti-SULT1A3 (IP:19499-1-AP, 4ug; Detection:19499-1-AP 1:200) with COLO 320 cells lysate 2240ug.

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  IHC staining of human hepatocirrhosis using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 19499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, Caco-2-Zellen, COLO 320-Zellen, HepG2-Zellen, HuH-7-Zellen, Mauslebergewebe, MCF-7-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

19499-1-AP bindet in WB, IP, IHC, ELISA SULT1A3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: NM_177552
• Gene symbol: SULT1A3
• Gene ID (NCBI): 6818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SULT1A3, also named as STM and SULT1A4, belongs to the sulfotransferase 1 family. SULT1A3 catalyzes the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.

Publikationen

Species	Application	Title
human	IHC	Cancer Lett; Expression of sulfotransferases and sulfatases in human breast cancer: impact on resveratrol metabolism.; Authors - Miksits Michaela M
mouse	WB	J Adv Res; Endogenous hydrogen sulfide improves vascular remodeling through PPARδ/SOCS3 signaling.; Authors - Danyang Tian