STRN Polyklonaler Antikörper

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• KD/KO Validated

STRN Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 21624-1-AP

Synonyme

SG2NA, Striatin, STRN

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Galerie der Validierungsdaten

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  WB analysis using 21624-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21624-1-AP (STRN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21624-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21624-1-AP (STRN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21624-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21624-1-AP (STRN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21624-1-AP

  IP result of anti-STRN(IP:21624-1-AP, 4ug; Detection:21624-1-AP 1:4000) with mouse brain tissue lysate 1080 ug.

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  IHC staining of mouse brain using 21624-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21624-1-AP (STRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 21624-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21624-1-AP (STRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 21624-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21624-1-AP (STRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21624-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21624-1-AP (STRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21624-1-AP

  Immunofluorescent analysis of Hela cells, using STRN antibody 21624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 21624-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human STRN (21624-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

21624-1-AP bindet in WB, IP, IHC, IF, FC, ELISA STRN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STRN fusion protein Ag15984
• Vollständiger Name: striatin, calmodulin binding protein
• Berechnetes Molekulargewicht: 780 aa, 86 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC106879
• Gene symbol: STRN
• Gene ID (NCBI): 6801
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STRN (Striatin), a 780-amino acid protein, was identified and cloned more than a decade ago. In adult mammals, striatin is mainly expressed in neurons of both the central and peripheral nervous systems with very high levels of expression in the striatum, hence its name. STRN-ALK fusion may be a potential therapeutic target in the aggressive forms of thyroid cancer (PMID: 24475247).

Publikationen

Species	Application	Title
mouse	WB,IF	Front Cell Dev Biol; Striatin Is Required for Hearing and Affects Inner Hair Cells and Ribbon Synapses.; Authors - Prathamesh T Nadar-Ponniah ; KO Validated
human	WB	J Biol Chem; Heavy Metal-Induced Metallothionein Expression is Regulated by Specific Protein Phosphatase 2A Complexes.; Authors - Liping Chen
rat	WB	Sci Rep; Precise and systematic survey of the efficacy of multicomponent drugs against functional dyspepsia.; Authors - Junying Wei
human	WB,IHC	Biomed Res Int; High STRN Expression Promotes HCC Invasion and Migration but Not Cell Proliferation or Apoptosis through Facilitating Epithelial-Mesenchymal Transition.; Authors - Qian-Yu Du ; KD Validated
rat	WB	Genes (Basel); Effect of Combined Methamphetamine and Oxycodone Use on the Synaptic Proteome in an In Vitro Model of Polysubstance Use; Authors - Daniel Meyer
human,mouse	WB	Kaohsiung J Med Sci; CircTNPO3 promotes hepatocellular carcinoma progression by sponging miR-199b-5p and regulating STRN expression; Authors - Jing Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vicki (Verified Customer) (02-21-2022)

Great antibody for cell lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary rat neurons