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STOML2 Monoklonaler Antikörper
STOML2 Monoklonal Antikörper für IHC, IP, WB, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
WB, IP, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
1A2E9
Kat-Nr. : 60052-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Hausschwein-Hirngewebe, HepG2-Zellen, MCF-7-Zellen, RAW 264.7-Zellen |
Erfolgreiche IP | Maushirngewebe |
Erfolgreiche Detektion in IHC | humanes Magenkrebsgewebe, humanes Endometriumkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 4 publications below |
IHC | See 3 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Produktinformation
60052-1-Ig bindet in WB, IP, IHC, IF, ELISA STOML2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Maus / IgG2b |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | STOML2 fusion protein Ag0363 |
Vollständiger Name | stomatin (EPB72)-like 2 |
Berechnetes Molekulargewicht | 356 aa, 39 kDa |
Beobachtetes Molekulargewicht | 39 kDa |
GenBank-Zugangsnummer | BC002442 |
Gene symbol | STOML2 |
Gene ID (NCBI) | 30968 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for STOML2 antibody 60052-1-Ig | Protokoll herunterladen |
IHC protocol for STOML2 antibody 60052-1-Ig | Protokoll herunterladen |
IP protocol for STOML2 antibody 60052-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.
| ||
Mol Cell Proteomics An informatics-assisted label-free approach for personalized tissue membrane proteomics: case study on colorectal cancer. | ||
Int J Mol Sci The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer. | ||
Int J Cancer Novel prognostic protein markers of resectable pancreatic cancer identified by coupled shotgun and targeted proteomics using formalin-fixed paraffin-embedded tissues. | ||
Cell Death Dis Stomatin-like protein 2 regulates survivin expression in non-small cell lung cancer cells through β-catenin signaling pathway.
| ||
J Cell Sci Deficiency of PHB complex impairs respiratory supercomplex formation and activates mitochondrial flashes.
|