Stathmin 1 Monoklonaler Antikörper

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• KD/KO Validated

Stathmin 1 Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

CloneNo.

7D1E1

Kat-Nr. : 66090-1-Ig

Synonyme

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 66090-1-Ig

  WB result of Stathmin antibody (66090-1-Ig, 1:4000) with si-Control and si-Stathmin transfected Jurkat cells.

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  WB analysis of Jurkat using 66090-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66090-1-Ig (Stathmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66090-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66090-1-Ig (Stathmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66090-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66090-1-Ig (Stathmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 66090-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66090-1-Ig (Stathmin antibody at dilution of 1:50.

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  IF Staining of mouse olfactory epithelium tissue using 66090-1-Ig

  Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of STMN1 antibody (66090-1-IG, 1:200). Basal cell staining and dendritic processes as expected. By Dr. Brian Lin (Schwob Lab).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Hirngewebe, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Riechepithelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66090-1-Ig bindet in WB, IHC, IF,ELISA Stathmin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Stathmin 1 fusion protein Ag19102
• Vollständiger Name: stathmin 1/oncoprotein 18
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC014353
• Gene symbol: STMN1
• Gene ID (NCBI): 3925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.