MST1 Monoklonaler Antikörper

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• KD/KO Validated

MST1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2G11C1

Kat-Nr. : 66663-1-Ig

Synonyme

DKFZp686A2068, KRS2, MST 1, MST1, serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 66663-1-Ig

  WB result of MST1 antibody (66663-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MST1 transfected HepG2 cells.

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  WB analysis using 66663-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66663-1-Ig (MST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66663-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66663-1-Ig (MST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66663-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66663-1-Ig (MST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 66663-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using MST1 antibody (66663-1-Ig, Clone: 2G11C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

66663-1-Ig bindet in WB, IHC, IF, ELISA MST1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MST1 fusion protein Ag17738
• Vollständiger Name: serine/threonine kinase 4
• Berechnetes Molekulargewicht: 487 aa, 56 kDa
• Beobachtetes Molekulargewicht: 52-56 kDa
• GenBank-Zugangsnummer: BC093768
• Gene symbol: STK4
• Gene ID (NCBI): 6789
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.

Publikationen

Species	Application	Title
human	WB	Biofactors; FERM domain-containing protein 6 exerts a tumor-inhibiting role in thyroid cancer by antagonizing oncogenic YAP1; Authors - Wei Wang
human	WB	J Biol Chem; Angiomotins stimulate LATS kinase autophosphorylation and act as scaffolds that promote Hippo signaling.; Authors - Sebastian Mana-Capelli
mouse	IHC	J Endocr Soc; Effect of Inactivation of Mst1 and Mst2 in the Mouse Adrenal Cortex; Authors - Nour Abou Nader