STK32A Monoklonaler Antikörper

STK32A Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

1H2A6

Kat-Nr. : 67528-1-Ig

Synonyme

serine/threonine kinase 32A, STK32A, YANK1

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Galerie der Validierungsdaten

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  WB analysis of A549 using 67528-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67528-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67528-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 67528-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of JAR using 67528-1-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 67528-1-Ig (STK32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67528-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67528-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67528-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67528-1-Ig (STK32A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, JAR-Zellen, MCF-7-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67528-1-Ig bindet in WB, IHC,ELISA STK32A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STK32A fusion protein Ag10942
• Vollständiger Name: serine/threonine kinase 32A
• Berechnetes Molekulargewicht: 396aa,46 kDa; 166aa,20 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC021666
• Gene symbol: STK32A
• Gene ID (NCBI): 202374
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.