STIM2 Polyklonaler Antikörper

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• KD/KO Validated

STIM2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 21192-1-AP

Synonyme

KIAA1482, STIM2, stromal interaction molecule 2

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Galerie der Validierungsdaten

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  WB analysis using 21192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21192-1-AP (STIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21192-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21192-1-AP (STIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21192-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21192-1-AP (STIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21192-1-AP

  IP Result of anti-STIM2 (IP:21192-1-AP, 3ug; Detection:21192-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of mouse spleen using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21192-1-AP (STIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21192-1-AP (STIM2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21192-1-AP (STIM2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 21192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 21192-1-AP (STIM2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, humanes Hirngewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

21192-1-AP bindet in WB, IP, IHC, IF, ELISA STIM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STIM2 fusion protein Ag15530
• Vollständiger Name: stromal interaction molecule 2
• Berechnetes Molekulargewicht: 754 aa, 85 kDa
• Beobachtetes Molekulargewicht: 85-115 kDa
• GenBank-Zugangsnummer: BC146661
• Gene symbol: STIM2
• Gene ID (NCBI): 57620
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STIM2, also named as KIAA1482, functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. STIM2 may inhibit STIM1-mediated Ca2+ influx. STIM2 has 2 isoforms 105kd and 115kd with Differential levels of phosphorylation.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.; Authors - Ao Hu ; KO Validated
human,mouse	WB,IF	Cell Mol Life Sci; STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons; Authors - Karolina Serwach ; KD Validated
human	WB	Oncotarget; The calcium channel proteins ORAI3 and STIM1 mediate TGF-β induced Snai1 expression.; Authors - Atrayee Bhattacharya ; KD Validated
mouse	WB	Neurobiol Dis; Inhibiting store-operated calcium entry attenuates white matter secondary degeneration following SCI.; Authors - Ben C Orem
		Biochem J; STIM1 negatively regulates Ca2+ release from the sarcoplasmic reticulum in skeletal myotubes.; Authors - Lee Keon Jin KJ
mouse	WB	Cell Signal; RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.; Authors - Menbere Wendimu ; KD Validated