STC1 Polyklonaler Antikörper

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• KD/KO Validated

STC1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 20621-1-AP

Synonyme

stanniocalcin 1, STC, STC 1, STC1

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Galerie der Validierungsdaten

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  WB analysis of mouse small intestine using 20621-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20621-1-AP (STC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 20621-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20621-1-AP (STC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Produktinformation

20621-1-AP bindet in WB, IP, IHC, Cell treatment, ELISA STC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STC1 fusion protein Ag14697
• Vollständiger Name: stanniocalcin 1
• Berechnetes Molekulargewicht: 247 aa, 28 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC029044
• Gene symbol: STC1
• Gene ID (NCBI): 6781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STC1, also named as stanniocalcin 1, a 56kDa homodimeric glycoprotein hormone, is highly expressed in a variety of cancers such as breast, ovarian, and colorectal cancers (PMID: 27459971). This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC1 is implicated in several pathologies including retinal degeneration, cerebral ischemia, angiogenesis and inflammation (PMID: 25391215). The calculated molecular weight of STC1 is usually approximately 28 kda.

Publikationen

Species	Application	Title
human,mouse	IHC	Inflamm Bowel Dis; Pro-inflammatory Cytokines Promote the Occurrence and Development of Colitis-associated Colorectal Cancer by Inhibiting miR-615-5p; Authors - Danping Sun
human	WB	Life Sci; Adiponectin alleviates the symptoms of ischemic renal disease by inhibiting renal cell apoptosis.; Authors - Xiaotong Zheng
human	WB	Cancer Cell Int; PMID: 31889892; Authors - Chuanfeng Zhang
human	WB	Neurochem Res; Stanniocalcin-1 Protected Astrocytes from Hypoxic Damage Through the AMPK Pathway.; Authors - Binda Sun ; KD Validated
human	IHC	Oncol Lett; Stanniocalcin 1 is a prognostic biomarker in glioma.; Authors - Weijian Luo
human	WB	Cancer Biother Radiopharm; Sevoflurane Suppresses Glioma Cell Proliferation, Migration, and Invasion Both In Vitro and In Vivo Partially Via Regulating KCNQ1OT1/miR-146b-5p/STC1 Axis.; Authors - Jian Wen