STAT6 Polyklonaler Antikörper

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• KD/KO Validated

STAT6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 51073-1-AP

Synonyme

D12S1644, IL 4 STAT, STAT6, STAT6B, STAT6C

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 51073-1-AP

  WB result of STAT6 antibody (51073-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected NIH/3T3 cells.

• 

  WB analysis of NIH/3T3 using 51073-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 51073-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Kolonkarzinomgewebe, humanes Lungengewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Produktinformation

51073-1-AP bindet in WB, RIP, IHC, IF, ELISA STAT6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: signal transducer and activator of transcription 6, interleukin-4 induced
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC075852
• Gene symbol: STAT6
• Gene ID (NCBI): 6778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. The antibody could identify about 100 kDa band, similar to the reported in the literature(PMID: 10961983).

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; miR-182 targeting reprograms tumor-associated macrophages and limits breast cancer progression.; Authors - Chengxin Ma
human,mouse	WB	Cell Rep; Macrophage autophagy deficiency-induced CEBPB accumulation alleviates atopic dermatitis via impairing M2 polarization; Authors - Yongcheng Zhu
human	IF	Front Cell Infect Microbiol; Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.; Authors - Tian Luo
human	WB	Front Cell Dev Biol; A miR-129-5P/ARID3A Negative Feedback Loop Modulates Diffuse Large B Cell Lymphoma Progression and Immune Evasion Through Regulating the PD-1/PD-L1 Checkpoint; Authors - Weili Zheng
human	WB	Cells; Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.; Authors - Damian Kołat
mouse,rat	WB,IHC	Life Sci; Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis.; Authors - Tao Yang