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STAT3 Monoklonaler Antikörper
STAT3 Monoklonal Antikörper für IF
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human, Maus, Ratte
Anwendung
IF
Konjugation
CoraLite®594 Fluorescent Dye
CloneNo.
3G2D12
Kat-Nr. : CL594-60199
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in IF | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Immunfluoreszenz (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL594-60199 bindet in IF STAT3 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Maus / IgG2a |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | STAT3 fusion protein Ag0360 |
Vollständiger Name | signal transducer and activator of transcription 3 (acute-phase response factor) |
Berechnetes Molekulargewicht | 770 aa, 88 kDa |
Beobachtetes Molekulargewicht | 88 kDa |
GenBank-Zugangsnummer | BC000627 |
Gene symbol | STAT3 |
Gene ID (NCBI) | 6774 |
Konjugation | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3. |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.
Protokolle
Produktspezifische Protokolle | |
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IF protocol for CL594 STAT3 antibody CL594-60199 | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |