STAR Monoklonaler Antikörper

STAR Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1G6E6

Kat-Nr. : 67130-1-Ig

Synonyme

STAR, STARD1

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Galerie der Validierungsdaten

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  WB analysis of pig adrenal gland using 67130-1-Ig

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat adrenal gland using 67130-1-Ig

  rat adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 67130-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67130-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 67130-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67130-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse testis using 67130-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400, CoraLite®555 TNP1 antibody (CL555-17178, orange), CoraLite® Plus 488 BOULE antibody (CL488-13720, green).

• 

  IF Staining of mouse testis using 67130-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse testis using 67130-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Nebennierengewebe, humanes Hodengewebe, K-562-Zellen, Rattenhodengewebe, Ratten-Nebennierengewebe, THP-1-Zellen
• Erfolgreiche Detektion in IF: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

67130-1-Ig bindet in WB, IHC, IF, ELISA STAR und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Hausschwein, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STAR fusion protein Ag28698
• Vollständiger Name: steroidogenic acute regulatory protein
• Berechnetes Molekulargewicht: 285 aa, 32 kDa
• Beobachtetes Molekulargewicht: 28-32 kDa
• GenBank-Zugangsnummer: BC010550
• Gene symbol: STAR
• Gene ID (NCBI): 6770
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue

Publikationen

Species	Application	Title
mouse	IF	Mol Cell Endocrinol; HE4 overexpression in mice leads to leydig cell hyperplasia and spermatogensis impairment: Pathological implications for oligospermia; Authors - Pengyun Ling
mouse	IHC,WB	Toxicol Appl Pharmacol; Reproductive effects of pubertal exposure to neonicotinoid thiacloprid in immature male mice; Authors - Yong Zou
pig	IF	Theriogenology; Impacts of elevated temperature on morphology, oxidative stress levels, and testosterone synthesis in ex vivo cultured porcine testicular tissue; Authors - Kai Wang