STAC3 Polyklonaler Antikörper

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• KD/KO Validated

STAC3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 20392-1-AP

Synonyme

SH3 and cysteine rich domain 3, STAC3

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Galerie der Validierungsdaten

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  WB analysis using 20392-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20392-1-AP (STAC3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 20392-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 20392-1-AP (STAC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 20392-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 20392-1-AP (STAC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 20392-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20392-1-AP (STAC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 20392-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20392-1-AP (STAC3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse skeletal muscle using 20392-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using STAC3 antibody (20392-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse skeletal muscle using 20392-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using STAC3 antibody (20392-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

20392-1-AP bindet in WB, IHC, IF, CoIP, ELISA STAC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STAC3 fusion protein Ag14232
• Vollständiger Name: SH3 and cysteine rich domain 3
• Berechnetes Molekulargewicht: 364 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42-55 kDa
• GenBank-Zugangsnummer: BC008069
• Gene symbol: STAC3
• Gene ID (NCBI): 246329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,CoIP	Hum Mutat; STAC3 variants cause a congenital myopathy with distinctive dysmorphic features and malignant hyperthermia susceptibility.; Authors - Irina Zaharieva
mouse	WB	Structure; Multiple Sequence Variants in STAC3 Affect Interactions with CaV1.1 and Excitation-Contraction Coupling.; Authors - Britany Rufenach ; KO Validated
mouse	WB	Skelet Muscle; The SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscle.; Authors - Xiaofei Cong ; KO Validated
human	IF	J Cell Physiol; STAC3 incorporation into skeletal muscle triads occurs independent of the dihydropyridine receptor.; Authors - Marta Campiglio
human	WB	J Gen Physiol; Calcium current modulation by the γ1 subunit depends on alternative splicing of CaV1.1.; Authors - Yousra El Ghaleb
rat	WB	J Appl Physiol (1985); Preconditioning contractions prevent prolonged force depression and Ca2+-dependent proteolysis of STAC3 after damaging eccentric contractions.; Authors - Yuki Ashida