ST7L Polyklonaler Antikörper

ST7L Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17567-1-AP

Synonyme

FAM4B, ST7 related protein, ST7L, ST7R, STLR

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 17567-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17567-1-AP (ST7L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17567-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17567-1-AP (ST7L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 17567-1-AP

  IP Result of anti-ST7L (IP:17567-1-AP, 3ug; Detection:17567-1-AP 1:1500) with mouse testis tissue lysate 5000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, MCF-7-Zellen
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

17567-1-AP bindet in WB, IP, CoIP, ELISA ST7L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ST7L fusion protein Ag11768
• Vollständiger Name: suppression of tumorigenicity 7 like
• Berechnetes Molekulargewicht: 575 aa, 65 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: BC065501
• Gene symbol: ST7L
• Gene ID (NCBI): 54879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST7 (suppressor of tumorigenicity 7) gene has been proposed to be a tumor suppressor gene, product of which is a multi-pass membrane protein belonging to the ST7 family. ST7L (suppressor of tumorigenicity 7 protein-like) gene encodes a multi-pass membrane protein also belonging to the ST7 family, 8 isoforms produced by alternative splicing have been described . However, the molecular function of this gene product has not been determined so far.

Publikationen

Species	Application	Title
human	WB,CoIP	Cell Death Dis; MicroRNA-23b functions as an oncogene and activates AKT/GSK3β/β-catenin signaling by targeting ST7L in hepatocellular carcinoma.; Authors - Likun Zhuang
human	WB	J Exp Clin Cancer Res; Long noncoding RNA MIR31HG inhibits hepatocellular carcinoma proliferation and metastasis by sponging microRNA-575 to modulate ST7L expression.; Authors - Shaoying Yan
human	WB	Int J Mol Med; miR-378 functions as an onco-miRNA by targeting the ST7L/Wnt/β-catenin pathway in cervical cancer.; Authors - Shengjie Li