SSTR1 Polyklonaler Antikörper

SSTR1 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20587-1-AP

Synonyme

somatostatin receptor 1, Somatostatin receptor type 1, SRIF 2, SS 1 R, SS1 R, SS1R, SSTR1

---

Galerie der Validierungsdaten

• 

  WB analysis of L02 using 20587-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 20587-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20587-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20587-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 20587-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  FC experiment of SH-SY5Y using 20587-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug SSTR1 antibody (20587-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, BxPC-3-Zellen, Maus-Dünndarmgewebe, Maushirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20587-1-AP bindet in WB, FC, ELISA SSTR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SSTR1 fusion protein Ag14287
• Vollständiger Name: somatostatin receptor 1
• Berechnetes Molekulargewicht: 391 aa, 43 kDa
• Beobachtetes Molekulargewicht: 53 kDa, 63 kDa
• GenBank-Zugangsnummer: BC035618
• Gene symbol: SSTR1
• Gene ID (NCBI): 6751
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.