Somatostatin (64-81aa) Polyklonaler Antikörper

Somatostatin (64-81aa) Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17512-1-AP

Synonyme

SMST, somatostatin, Somatostatin (64-81aa), SST

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Galerie der Validierungsdaten

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  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:25600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17512-1-AP (Somatostatin (64-81aa) antibody) at dilution of 1:25600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17512-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP Somatostatin-Specific antibody (green) and 66198-1-Ig Insulin antibody (red) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 17512-1-AP (Somatostatin-Specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Somatostatin (64-81aa) antibody (17512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Somatostatin (64-81aa) antibody (17512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Somatostatin (64-81aa) antibody (17512-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe, humanes Pankreaskarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Pankreasgewebe, Maushirngewebe, Maus-Pankreasgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:6400-1:25600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

17512-1-AP bindet in IHC, IF, ELISA Somatostatin (64-81aa) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: somatostatin
• Berechnetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: NM_001048
• Gene symbol: SST
• Gene ID (NCBI): 6750
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. This antibody can recognize the full length and propeptide of Somatostatin.

Publikationen

Species	Application	Title
rat	IF	Aging Dis; PMID: 31440379; Authors - Fei Han
	IF	Cell Death Dis; Inhibition of Notch activity promotes pancreatic cytokeratin 5-positive cell differentiation to beta cells and improves glucose homeostasis following acute pancreatitis.; Authors - Xiaoyi Zhang