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SSRP1 Monoklonaler Antikörper

SSRP1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D3H4

Kat-Nr. : CL488-67313

Synonyme

FACT, FACT 80 kDa subunit, FACT complex subunit SSRP1, FACT80, FACTp80, hSSRP1, SSRP1, T160

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SSRP1 Monoclonal ant (CL488-67313)

    IF Staining of HepG2 using CL488-67313

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SSRP1 antibody (CL488-67313, Clone: 3D3H4 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated SSRP1 Monoclonal ant (CL488-67313)

    FC experiment of HeLa using CL488-67313

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SSRP1 (CL488-67313, Clone:3D3H4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67313 bindet in IF, FC (Intra) SSRP1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen SSRP1 fusion protein Ag8318
Vollständiger Name structure specific recognition protein 1
Berechnetes Molekulargewicht 81 kDa
GenBank-ZugangsnummerBC005116
Gene symbol SSRP1
Gene ID (NCBI) 6749
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 SSRP1 antibody CL488-67313Protokoll herunterladen
FC protocol for CL Plus 488 SSRP1 antibody CL488-67313Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen