SRY Monoklonaler Antikörper

SRY Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2E9A4

Kat-Nr. : 67349-1-Ig

Synonyme

sex determining region Y, SRY, TDF, TDY, Testis determining factor

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Galerie der Validierungsdaten

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  WB analysis of LNCaP using 67349-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67349-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SRY negative cell line HeLa cell line was served as negative control.

• 

  WB analysis of DU 145 using 67349-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, DU 145-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67349-1-Ig bindet in WB, ELISA SRY und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SRY fusion protein Ag12610
• Vollständiger Name: sex determining region Y
• Berechnetes Molekulargewicht: 204 aa, 24 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC074924
• Gene symbol: SRY
• Gene ID (NCBI): 6736
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.

Publikationen

Species	Application	Title
human	WB	Cell Rep; Recruitment of DNA to tumor-derived microvesicles.; Authors - James W Clancy