SRY Polyklonaler Antikörper

SRY Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17930-1-AP

Synonyme

sex determining region Y, SRY, TDF, TDY, Testis determining factor

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Galerie der Validierungsdaten

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  WB analysis of human testis using 17930-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17930-1-AP (SRY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17930-1-AP (SRY antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17930-1-AP (SRY antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Hodengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17930-1-AP bindet in WB, IHC,ELISA SRY und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRY fusion protein Ag12360
• Vollständiger Name: sex determining region Y
• Berechnetes Molekulargewicht: 204 aa, 24 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC074924
• Gene symbol: SRY
• Gene ID (NCBI): 6736
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.