SRR Polyklonaler Antikörper

SRR Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 17955-1-AP

Synonyme

ILV1, ISO1, serine racemase, SRR

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 17955-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17955-1-AP (SRR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17955-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17955-1-AP (SRR Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17955-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17955-1-AP (SRR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17955-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17955-1-AP (SRR Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17955-1-AP

  IP result of anti-SRR (IP:17955-1-AP, 4ug; Detection:17955-1-AP 1:600) with mouse brain tissue lysate 4000 ug.

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  IHC staining of human kidney using 17955-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17955-1-AP (SRR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17955-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17955-1-AP (SRR Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 17955-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17955-1-AP (SRR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 17955-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17955-1-AP (SRR Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

17955-1-AP bindet in WB, IP, IHC, ELISA SRR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRR fusion protein Ag12449
• Vollständiger Name: serine racemase
• Berechnetes Molekulargewicht: 340 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC074728
• Gene symbol: SRR
• Gene ID (NCBI): 63826
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRR(serine racemase) is a 37 kDa protein and belongs to the serine/threonine dehydratase family. It plays a role in neuronal activity and a potential biological role in the regulation of N-methyl-D-aspartate (NMDA) receptor activity in these peripheral organs as well. SRR catalyzes the racemization between l- and d-serine.

Publikationen

Species	Application	Title
mouse	WB	J Neurosci; Selective ablation of Sod2 in astrocytes induces sex-specific effects on cognitive function, D-serine availability, and astrogliosis.; Authors - Matthew P Baier