SRP19 Polyklonaler Antikörper

SRP19 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16033-1-AP

Synonyme

SRP19

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Galerie der Validierungsdaten

• 

  WB analysis of human liver using 16033-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16033-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16033-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 16033-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 16033-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16033-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16033-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16033-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16033-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16033-1-AP (SRP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16033-1-AP

  IP Result of anti-SRP19 (IP:16033-1-AP, 4ug; Detection:16033-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IF Staining of HeLa using 16033-1-AP

  Immunofluorescent analysis of HeLa cells using 16033-1-AP (SRP19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, A549-Zellen, HeLa-Zellen, K-562-Zellen, Maus-Eierstockgewebe, Mauslebergewebe, Mausnierengewebe, Raji-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

16033-1-AP bindet in WB, IP, IF, ELISA SRP19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRP19 fusion protein Ag8903
• Vollständiger Name: signal recognition particle 19kDa
• Berechnetes Molekulargewicht: 144 aa, 16 kDa
• Beobachtetes Molekulargewicht: 18-25 kDa
• GenBank-Zugangsnummer: BC010947
• Gene symbol: SRP19
• Gene ID (NCBI): 6728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The signal recognition particle (SRP) is one of the few functional small RNP particles. The SRP couples the synthesis of membrane and secretory proteins across or into the endoplasmic reticulum (ER) membrane in eukaryotes, as well as across the bacterial plasma membrane, and chloroplast thylakoid membranes. The mammalian SRP is composed of a 7S (or 7SL) RNA and six different proteins, SRP9, SRP14, SRP19, SRP54, SRP68 and SRP72. All of the components of SRP, including SRP RNA, participate directly in the overall protein targeting process. SRP19 binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. SRP19 was shown to significantly enhance SRP54 attachment to helix 8 of 7SL RNA. Binding of SRP19 leads to restructuring of both helix 6 and 8, causing local changes at the SRP54-binding site. This antibody is a rabbit polyclonal antibody raised against full length SRP19 of human origin.

Publikationen

Species	Application	Title
	WB	Nucleic Acids Res; The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins.; Authors - Joori Park
human	WB	Nucleic Acids Res; SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.; Authors - Anne-Sophie Gribling-Burrer
human	WB	PLoS One; The CELF1 RNA-Binding Protein Regulates Decay of Signal Recognition Particle mRNAs and Limits Secretion in Mouse Myoblasts.; Authors - Joseph Russo
human	WB	Elife; The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1.; Authors - Diego Acosta-Alvear