Spermidine synthase Polyklonaler Antikörper

Spermidine synthase Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 19858-1-AP

Synonyme

PAPT, SPDSY, Spermidine synthase, SPS1, SRM, SRML1

---

Galerie der Validierungsdaten

• 

  WB analysis using 19858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19858-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 19858-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 19858-1-AP

  IP Result of anti-Spermidine synthase (IP:19858-1-AP, 4ug; Detection:19858-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human kidney using 19858-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19858-1-AP (Spermidine synthase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19858-1-AP (Spermidine synthase antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 19858-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19858-1-AP (Spermidine synthase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19858-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Spermidine synthase antibody (19858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, Maushirngewebe, Mauslebergewebe, PC-3-Zellen, Rattenlebergewebe, RAW 264.7-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

19858-1-AP bindet in WB, IP, IHC, IF, ELISA Spermidine synthase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Spermidine synthase fusion protein Ag13936
• Vollständiger Name: spermidine synthase
• Berechnetes Molekulargewicht: 302 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000309
• Gene symbol: SRM
• Gene ID (NCBI): 6723
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.; Authors - Leslie A Sandusky-Beltran
human	WB,IF	Nat Commun; Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM.; Authors - Hongming Miao
human	WB	Cancer Sci; The miR-1908/SRM regulatory axis contributes to extracellular vesicle secretion in prostate cancer.; Authors - Fumihiko Urabe
mouse	WB	J Proteomics; Proteomic analysis revealed different responses to hypergravity of soleus and extensor digitorum longus muscles in mice.; Authors - Takashi Ohira
mouse	WB	Am J Physiol Cell Physiol; The Regulation of Polyamine Pathway Proteins in Models of Skeletal Muscle Hypertrophy and Atrophy - a potential role for mTORC1.; Authors - Michael Tabbaa
	IHC	Biol Reprod; Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period.; Authors - Katherine M Halloran