SQLE Monoklonaler Antikörper

SQLE Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Ratte und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1C9A2

Rezensionen (1)

Kat-Nr. : 67206-1-Ig

Synonyme

ERG1, FLJ30795, SE, SQLE, squalene epoxidase, Squalene monooxygenase

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Galerie der Validierungsdaten

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  WB analysis of A549 using 67206-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67206-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67206-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67206-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 67206-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 67206-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SQLE antibody (67206-1-Ig, Clone: 1C9A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HepG2-Zellen, L02-Zellen
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

67206-1-Ig bindet in WB, IF, ELISA SQLE und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SQLE fusion protein Ag3266
• Vollständiger Name: squalene epoxidase
• Berechnetes Molekulargewicht: 574 aa, 64 kDa
• Beobachtetes Molekulargewicht: 50-64 kDa
• GenBank-Zugangsnummer: BC017033
• Gene symbol: SQLE
• Gene ID (NCBI): 6713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)

Publikationen

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC; Authors - Xinyuan Zhao
mouse	IF	Cell Signal; Knockdown of SQLE promotes CD8+ T cell infiltration in the tumor microenvironment; Authors - Jun Wu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (11-24-2022)

Detected a band around the expected molecular weight, but with some other background bands.

• Applications: Western Blot