SPZ1 Polyklonaler Antikörper

SPZ1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18931-1-AP

Synonyme

NYD TSP1, spermatogenic leucine zipper 1, SPZ1, Testis specific protein 1, TSP1

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 18931-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 18931-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 18931-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of PC-3 using 18931-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 18931-1-AP (SPZ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, DU 145-Zellen, humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18931-1-AP bindet in WB, IHC, IF, ELISA SPZ1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPZ1 fusion protein Ag6995
• Vollständiger Name: spermatogenic leucine zipper 1
• Berechnetes Molekulargewicht: 430 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49-55 kDa
• GenBank-Zugangsnummer: BC033798
• Gene symbol: SPZ1
• Gene ID (NCBI): 84654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.