SPTLC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SPTLC1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 15376-1-AP

Synonyme

HSAN, HSAN1, HSN1, LBC1, LCB 1, LCB1, Serine palmitoyltransferase 1, SPT 1, SPT1, SPTI, SPTLC1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 15376-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15376-1-AP

  WB result of SPTLC1 antibody (15376-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC1 transfected HEK-293 cells.

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  WB analysis of HepG2 using 15376-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15376-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15376-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15376-1-AP

  IP Result of anti-SPTLC1 (IP:15376-1-AP, 4ug; Detection:15376-1-AP 1:500) with HEK-293 cells lysate 880ug.

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  IHC staining of human colon cancer using 15376-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15376-1-AP (SPTLC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 15376-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15376-1-AP (SPTLC1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Lebergewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below

Produktinformation

15376-1-AP bindet in WB, IP, IHC, ELISA SPTLC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPTLC1 fusion protein Ag1162
• Vollständiger Name: serine palmitoyltransferase, long chain base subunit 1
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC007085
• Gene symbol: SPTLC1
• Gene ID (NCBI): 10558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).

Publikationen

Species	Application	Title
rat	IHC	Cell Metab; Central Ceramide Signaling Mediates Obesity-Induced Precocious Puberty.; Authors - Violeta Heras
human	WB	Sci Adv; Inhibition of sphingolipid de novo synthesis counteracts muscular dystrophy.; Authors - Pirkka-Pekka Laurila
mouse	WB	J Neurosci; MicroRNA-137/181c regulates serine palmitoyltransferase and in turn amyloid β, novel targets in sporadic Alzheimer's disease.; Authors - Hirosha Geekiyanage
mouse	WB,IHC	Neurobiol Aging; Inhibition of serine palmitoyltransferase reduces Aβ and tau hyperphosphorylation in a murine model: a safe therapeutic strategy for Alzheimer's disease.; Authors - Hirosha Geekiyanage
mouse	WB	Am J Physiol Lung Cell Mol Physiol; Neutrophil elastase increases airway ceramide levels via upregulation of serine palmitoyltransferase.; Authors - Sophia Karandashova
human	WB	Front Cell Neurosci; Activation of Mitochondrial Unfolded Protein Response in SHSY5Y Expressing APP Cells and APP/PS1 Mice.; Authors - Yang Shen ; KD Validated