SPTBN1-Specific Polyklonaler Antikörper

SPTBN1-Specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 19722-1-AP

Synonyme

Beta II spectrin, beta-fodrin, betaSpII, ELF, Fodrin beta chain, Spectrin beta chain, brain 1, SPTB2, SPTBN1, SPTBN1-Specific

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 19722-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 19722-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19722-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 19722-1-AP

  IP Result of anti-SPTBN1-Specific (IP:19722-1-AP, 5ug; Detection:19722-1-AP 1:600) with HEK-293 cells lysate 1000ug.

• 

  IHC staining of human liver cancer using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mauslungengewebe, Mausnierengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungengewebe, humanes Nierengewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

19722-1-AP bindet in WB, IP, IHC, IF, ELISA SPTBN1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: spectrin, beta, non-erythrocytic 1
• Berechnetes Molekulargewicht: 275 kDa
• Beobachtetes Molekulargewicht: 275 kDa
• GenBank-Zugangsnummer: NM_003128
• Gene symbol: SPTBN1
• Gene ID (NCBI): 6711
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.

Publikationen

Species	Application	Title
human	WB,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
human	WB,IHC	Proteomics; Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network.; Authors - Shen Chengpin C
human	IF	Cancer Med; MiR-362 suppresses cervical cancer progression via directly targeting BAP31 and activating TGFβ/Smad pathway.; Authors - Shuya Yang
mouse	IF	FEBS Open Bio; Identification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short-term exposure to tobacco smoke.; Authors - Kanako Niimori-Kita
mouse,human	WB	Neuron; Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development; Authors - Minjie Shen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ruchi (Verified Customer) (01-25-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse skin