SPTBN1 Polyklonaler Antikörper

SPTBN1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25681-1-AP

Synonyme

Beta II spectrin, betaSpII, ELF, Fodrin beta chain, Spectrin beta chain, brain 1, SPTB2, SPTBN1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 25681-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25681-1-AP (SPTBN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 25681-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25681-1-AP (SPTBN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25681-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25681-1-AP (SPTBN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 25681-1-AP

  Immunofluorescent analysis of HEK-293 cells using 25681-1-AP (SPTBN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HEK-293-Zellen, Mausherzgewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25681-1-AP bindet in WB, IF, ELISA SPTBN1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPTBN1 fusion protein Ag22459
• Vollständiger Name: spectrin, beta, non-erythrocytic 1
• Berechnetes Molekulargewicht: 2364 aa, 275 kDa
• Beobachtetes Molekulargewicht: 275 kDa
• GenBank-Zugangsnummer: BC137282
• Gene symbol: SPTBN1
• Gene ID (NCBI): 6711
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.