SPRED2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SPRED2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 24091-1-AP

Synonyme

Spred 2, SPRED2

---

Galerie der Validierungsdaten

• 

  WB analysis using 24091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24091-1-AP

  WB result of SPRED2 antibody (24091-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPRED2 transfected MCF-7 cells.

• 

  WB analysis of mouse testis using 24091-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24091-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 24091-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 24091-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24091-1-AP (SPRED2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24091-1-AP (SPRED2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24091-1-AP (SPRED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 24091-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24091-1-AP (SPRED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 24091-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24091-1-AP (SPRED2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, Maushirngewebe, Maushodengewebe, MCF-7-Zellen, Rattenhirngewebe, Rattenhodengewebe, T-47D-Zellen
• Erfolgreiche Detektion in IHC: Rattenhodengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

24091-1-AP bindet in WB, IHC, IF, ELISA SPRED2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPRED2 fusion protein Ag21359
• Vollständiger Name: sprouty-related, EVH1 domain containing 2
• Berechnetes Molekulargewicht: 418 aa, 48 kDa
• Beobachtetes Molekulargewicht: 40-48 kDa
• GenBank-Zugangsnummer: BC136334
• Gene symbol: SPRED2
• Gene ID (NCBI): 200734
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPRED2 is a member of the SPRED family of proteins that modulate GFR signaling by inhibiting the Ras-MAPK cascade (PMID: 17094949). SPRED family members are characterized by an N-terminal Enabled/VASP homology 1 domain (EVH1), a central c-Kit binding domain (KBD) and a C-terminal Sprouty-related domain (SPR) (PMID: 17691106). Three mammalian SPREDs (SPRED1-3) have been described. In adult tissues, SPRED2 is expressed ubiquitously, but most highly expressed in glandular epithelia (PMID: 15580519; 17691106). Reduced expression level of SPRED2 has been reported in human hepatocellular carcinoma (HCC), suggesting SPRED2 may play a role in tumorgenesis (PMID: 16652141). Gene disruption of SPRED2 causes dwarfism (PMID: 15946934).

Publikationen

Species	Application	Title
human	IHC	PLoS One; Expression of Spred2 in the urothelial tumorigenesis of the urinary bladder; Authors - Shinsuke Oda
mouse	WB	Biol Pharm Bull; Inhibition of Spred/Sprouty Expression in the Skin of a Contact Dermatitis-Like Model.; Authors - Hiroyasu Sakai
mouse	IF	Histol Histopathol; microRNA-141-3p mediates epithelial cell proliferation, apoptosis, and epithelial-mesenchymal transition and alleviates pulmonary fibrosis in mice via Spred2; Authors - Liang Zhu
human	WB	Int J Mol Sci; SPRED2: A Novel Regulator of Epithelial-Mesenchymal Transition and Stemness in Hepatocellular Carcinoma Cells; Authors - Tong Gao