SPON2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SPON2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 20513-1-AP

Synonyme

DIL 1, DIL1, M spondin, Mindin, SPON2, Spondin 2

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Galerie der Validierungsdaten

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  WB analysis of LNCaP using 20513-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 20513-1-AP (SPON2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20513-1-AP (SPON2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20513-1-AP (SPON2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Prostatahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

20513-1-AP bindet in WB, IHC, IF, ELISA SPON2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPON2 fusion protein Ag14337
• Vollständiger Name: spondin 2, extracellular matrix protein
• Berechnetes Molekulargewicht: 331 aa, 36 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC002707
• Gene symbol: SPON2
• Gene ID (NCBI): 10417
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPON2 (Spondin 2), also known as Mindin, is a member of the F-spondin family of secreted extracellular matrix proteins. This protein has been implicated in axon guidance, immune response and adhesion. SPON2 is essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (PMID: 14691481). It also functions as an integrin ligand for inflammatory cell recruitment and T-cell priming (PMID: 19153605). Besides, SPON2 has been reported as a prognostic biomarker for ovarian and prostate cancer (PMID: 17490732; 22615945; 23665271).

Publikationen

Species	Application	Title
human	WB	J Cell Mol Med; Spondin 2 promotes the proliferation, migration and invasion of gastric cancer cells.; Authors - Haoming Lu ; KD Validated
human,mouse	WB,IF	Front Cell Dev Biol; Transcriptional Activation of Matricellular Protein Spondin2 (SPON2) by BRG1 in Vascular Endothelial Cells Promotes Macrophage Chemotaxis.; Authors - Nan Li
human	IHC	Front Cell Dev Biol; Integrative Analysis of DNA Methylation and Transcriptome Identifies a Predictive Epigenetic Signature Associated With Immune Infiltration in Gliomas.; Authors - Jianlei Zhang
human	IHC	Front Oncol; Knockdown of SPON2 inhibits the growth of triple-negative breast cancer; Authors - Xueyi Hu