SPIN1-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SPIN1-Specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 19531-1-AP

Synonyme

OCR, Ovarian cancer related protein, SPIN, SPIN1, SPIN1-Specific, spindlin 1

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Galerie der Validierungsdaten

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  WB analysis of SKOV-3 using 19531-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 19531-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19531-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 19531-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 19531-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 19531-1-AP

  Immunofluorescent analysis of Hela cells, using SPIN1 antibody 19531-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, A2780-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

19531-1-AP bindet in WB, IHC, IF, ELISA SPIN1-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: spindlin 1
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: NM_006717
• Gene symbol: SPIN1
• Gene ID (NCBI): 10927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPIN1 (Spindlin 1) is a histone methylation reader, which recognizes trimethylated histone H3 lysine 4 through the protein-protein interaction. SPIN1 was initially found in ovarian cancer via searching ovarian cancer-related genes . It is overexpressed in ovarian cancer tissue, which exhibits a high homology to mouse Spin (Spindlin) gene. Signaling pathways responsible for SPIN1 functions include PI3K/Akt, Wnt and RET that are highly relevant to tumorigenesis.(PMID: 34492335, PMID: 31790762)

Publikationen

Species	Application	Title
human	WB	Oncotarget; Suppressive role exerted by microRNA-29b-1-5p in triple negative breast cancer through SPIN1 regulation.; Authors - Rosa Drago-Ferrante ; KD Validated
human	WB	J Cell Biochem; Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.; Authors - Sulpizio Marilisa M
human	IHC	Bioengineered; The microRNA-381(miR-381)/Spindlin1(SPIN1) axis contributes to cell proliferation and invasion of colorectal cancer cells by regulating the Wnt/β-catenin pathway.; Authors - Ling Zhou
human	IHC	J Exp Clin Cancer Res; SPIN1, negatively regulated by miR-148/152, enhances Adriamycin resistance via upregulating drug metabolizing enzymes and transporter in breast cancer.; Authors - Xu Chen