SPI1 Monoklonaler Antikörper

SPI1 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2H3D3

Kat-Nr. : 66618-2-Ig

Synonyme

31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1

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Galerie der Validierungsdaten

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  WB analysis of Daudi using 66618-2-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66618-2-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 66618-2-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66618-2-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 66618-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 66618-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Raji-Zellen, Rattenmilzgewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66618-2-Ig bindet in WB, IHC, IF, CoIP, ELISA SPI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: spleen focus forming virus (SFFV) proviral integration oncogene spi1
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 32-38 kDa
• GenBank-Zugangsnummer: NM_003120
• Gene symbol: SPI1
• Gene ID (NCBI): 6688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.

Publikationen

Species	Application	Title
human	IHC,WB	Discov Oncol; SPI1 is a prognostic biomarker of immune infiltration and immunotherapy efficacy in clear cell renal cell carcinoma; Authors - Huayi Feng
human	WB	Cancers (Basel); ACAP1 Deficiency Predicts Inferior Immunotherapy Response in Solid Tumors; Authors - Qiyi Yi
human	WB,IF,CoIP	Exp Cell Res; IRF1 suppresses colon cancer proliferation by reducing SPI1-mediated transcriptional activation of GPX4 and promoting ferroptosis; Authors - Yilin Chen