SPI1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SPI1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 55100-1-AP

Synonyme

31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1

---

Galerie der Validierungsdaten

• 

  WB analysis using 55100-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55100-1-AP (SPI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 55100-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 55100-1-AP (SPI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

55100-1-AP bindet in WB, IF, ELISA SPI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: spleen focus forming virus (SFFV) proviral integration oncogene spi1
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31-42 kDa
• GenBank-Zugangsnummer: NM_003120
• Gene symbol: SPI1
• Gene ID (NCBI): 6688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPI1 binds to the PU-box which is a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. SPI1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. It also binds RNA and may modulate pre-mRNA splicing. The antibody is specific to SPI1. SPI1 can be detected 37-42 kDa (PMID: 28362429, 31586032, 28586009).

Publikationen

Species	Application	Title
	IF	Mucosal Immunol; IL-33/ST2/IL-9/IL-9R signaling disrupts ocular surface barrier in allergic inflammation.; Authors - Jiaoyue Hu
mouse	WB	Sci Rep; Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.; Authors - Yanhong Shi
human	WB	J Leukoc Biol; miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression.; Authors - Hai-Shuang Lin ; KD Validated
human	WB	Med Oncol; PU.1 negatively regulates tumorigenesis in non-small-cell lung cancer; Authors - Xiaoxiao Liu
human	WB	Exp Cell Res; An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1; Authors - Xuanxuan Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Andrew (Verified Customer) (08-09-2021)

When used in our capillary electrophoresis equipment (JESS), the proteintech antibody for SPI1 seemed to show specific binding to a protein at around 60KD. We believe this may be recognising a dimerized form of the protein. This detection was consistent between lanes and responded as expected to varying amounts of sample protein and antibody concentration.

• Applications: Other
• Primary Antibody Dilution: Various
• Cell Tissue Type: Human brain tissue

SPI1 Antibody Other validation (Various dilution) in Human brain tissue (Cat no:55100-1-AP)