SPATA22 Polyklonaler Antikörper

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• KD/KO Validated

SPATA22 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (2)

Kat-Nr. : 16989-1-AP

Synonyme

NYD SP20, SPATA22, spermatogenesis associated 22

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16989-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 16989-1-AP

  IP Result of anti-SPATA22 (IP:16989-1-AP, 3ug; Detection:16989-1-AP 1:500) with mouse testis tissue lysate 4800ug.

• 

  IHC staining of mouse testis using 16989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 16989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, humanes Hodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 8 publications below

Produktinformation

16989-1-AP bindet in WB, IP, IHC, IF, ELISA SPATA22 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPATA22 fusion protein Ag10591
• Vollständiger Name: spermatogenesis associated 22
• Berechnetes Molekulargewicht: 363 aa, 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC029483
• Gene symbol: SPATA22
• Gene ID (NCBI): 84690
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spata22 (NYD-SP20), a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells. It is essential for one or more key events of early meiotic prophase, as homologous chromosomes of mutant germ cells do not achieve normal synapsis or repair meiotic DNA double-strand breaks.

Publikationen

Species	Application	Title
mouse	IF	Nucleic Acids Res; RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis; Authors - Chao Liu
mouse	IF	Nat Commun; Nutrient restriction synergizes with retinoic acid to induce mammalian meiotic initiation in vitro.; Authors - Xiaoyu Zhang
mouse	WB	Nat Commun; The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells.; Authors - Jingjing Zhang
mouse	IF	Nucleic Acids Res; SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.; Authors - Yongjuan Guan
mouse	IF	Cell Death Differ; A truncating variant of RAD51B associated with primary ovarian insufficiency provides insights into its meiotic and somatic functions.; Authors - Monica M Franca
mouse	WB	Nat Commun; MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination.; Authors - Mengcheng Luo ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Murali (Verified Customer) (03-11-2021)

Works nicely.Used on HeLa cells

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:25
• Cell Tissue Type: HeLa cells

SPATA22 Antibody Immunofluorescence validation (1:25 dilution) in HeLa cells (Cat no:16989-1-AP)

FH

Xiaoyu (Verified Customer) (11-04-2019)

good for immunofluorescence

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: germ cell